Carla is completely absent during this season. It is unknown what has happened to her, as she is never mentioned by the other characters at all. It's a possibility that she moved. Taxicrab has taken over her shop.
In this season, the characters do not talk to the viewers or ask them questions at all.
During the end credits, Bungo doesn't ask the viewers any questions relating to one of the episodes. Instead, the characters are seen doing various things.
A new song is introduced this season called "We Can Do It".
The characters designs are different than the last season. However, Bungo's Season 1 design is only shown when he carries the title card of each episode.
During the "Gotta Zip" song, it is sung by gentlemen instead of by the characters.
A new location has been introduced, called Mount Zipmore, which is a parody of Mount Rushmore.
The theme song is different than the first season. Zooter's line has been changed into “Coming through!” instead of "Got a message!".
Also, Toadhog smiled in the Season 2 intro unlike Season 1.